Heterologous protein transfer within structured myxobacteria biofilms

An age-structured continuum model for myxobacteria

Myxobacteria are social bacteria, that can glide in 2D and form counter-propagating, interacting waves. Here we present a novel age-structured, continuous macroscopic model for the movement of myxobacteria. The derivation is based on microscopic interaction rules that can be formulated as a particle-based model and set within the SOH (Self-Organized Hydrodynamics) framework. The strength of thi...

III.3. Evidence for horizontal gene transfer between eukaryotes and Myxobacteria Evidence for horizontal gene transfer from Eukaryotes to Myxobacteria

Hydrodynamic dispersion within porous biofilms.

Many microorganisms live within surface-associated consortia, termed biofilms, that can form intricate porous structures interspersed with a network of fluid channels. In such systems, transport phenomena, including flow and advection, regulate various aspects of cell behavior by controlling nutrient supply, evacuation of waste products, and permeation of antimicrobial agents. This study presen...

Gene transfer in microbial biofilms

Quantification and prediction of gene transfer in natural environments is required for reliable assessment of risks associated with the transfer of recombinant DNA in genetically engineered microbial inocula, and for a greater understanding of the mechanisms controlling transfer of genetic material. Published models of gene transfer describe plasmid transfer in homogeneous, liquid culture syste...

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...